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Commercial poultry production farms are under increasing pressure to reduce the use of antibiotics while maintaining health standards and pathogen control. Currently, there is an increased need for the development of effective products that serve as alternatives to antibiotics. We have developed a water applied biopromotor through careful selection of different types of prebiotics strategies (inactivated fermented Bacillus subtilis fragments and yeast cell wall extracts) that improves mucosal integrity and controls gram negative pathogens, while increasing productivity parameters and health status of the birds.