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Salmonella is the leading cause of foodborne infections and is a major public health concern worldwide. Poultry meat and eggs are a major reservoir of foodborne Salmonella serovars and vaccination against the pathogen is one of the important measures to curb infectious outbreaks and reduce antibiotic use, currently a major concern for consumers. In this study we evaluated the efficacy of a commercially available inactive, orally administered, subunit vaccine (Biotech Vac Salmonella) and determined if the immune response was protective and persistent (long-term immunity) against mobile Salmonella spp. in two separate commercial layer hen flocks (n=120,000 layers/lot) located in Pergamino, Buenos Aires, Argentina.