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Salmonella infection is a major cause of bacterial enteric illness in both humans and animals. This foodborne pathogen is often associated with poultry production and contaminated poultry products. Currently, vaccination is one of the main strategies to control Salmonella in commercial poultry farms avoiding the use of antimicrobials while improving food safety for consumers. We have previously tested the efficacy of a commercially available subunit vaccine (Biotech Vac Salmonella) to protect against Salmonella enteritidis.